RACE網路上都找不到繁體中文的介紹
找到的大都是廣告
Rapid Amplification of cDNA Ends , RACE我自己也發現
RACE, 5' RACE or Rapid Amplification of cDNA Ends, is a molecular biology technique used to amplify the 5' ends of cDNA by a special PCR reaction. In particular, it is used to clone 5' UTR fragments of specific cDNA, which are usually missed by regular cloning techniques. In order to conduct a RACE amplification, the sequence of a part of the cDNA of interest has to be known.
RACE-PCR requires a first round of cDNA extension by the enzyme terminal transferase, which adds a homopolymeric tail to the 5' end of all template cDNAs. A PCR reaction is then carried out, which uses a general sense primer that binds to the new 5' tail and a specific antisense primer that binds to the known cDNA sequence.
以生物學而言,
網路上繁體中文
深入相關資訊大都只是一些零碎片段
沒有像是大陸 生命經緯 那般全方位的專站
也許 中文化是有必要的
雖然可能會撕破某些人學問高深的面具...
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